To develop a simple, rapid, accurate, precise and reproducible reversed phase high performance liquid chromatographic method has been developed for the estimation of levofloxacin in bulk and in tablets. Development of a method for the determination of levofloxacin, an antibacterial agent, by RP-HPLC was undertaken using a new mobile phase of phosphate buffer:acetonitrile (40:60, v/v). The eluent was monitored at 295 nm. The optimized conditions developed showed a linear response from 10 to 50 μg/mL, with a correlation coefficient of 1.000. The retention time of the drug was found to be 2.675 min. The limit of detection (LOD) and limit of quantification (LOQ) were 0.015 and 0.051 μg/mL, respectively. The assay values for the two branded levofloxacin tablets tested were 100.0 and 98.2 %, respectively with % relative standard deviation (RSD) of 1.01 and 0.89, respectively. Recovery data were good. Placebo study for specificity and interference of common excipients showed that the method was specific and free from interfering substances. Therefore, the fully validated method developed was sensitive enough to carry out analysis of levofloxacin in tablet formulations with regard to its run time, simplicity of sample preparation and accuracy. The proposed method was statistically evaluated and can be applied for routine quality control analysis of levofloxacin in tablets.
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